Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4637Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 20ng/ml | 2.07 | 2.123 | 2.067 |
| 2.175 | |||
| 10ng/ml | 1.338 | 1.343 | 1.287 |
| 1.347 | |||
| 5ng/ml | 0.891 | 0.864 | 0.8090000000000001 |
| 0.837 | |||
| 2.5ng/ml | 0.406 | 0.429 | 0.373 |
| 0.451 | |||
| 1.25ng/ml | 0.29 | 0.277 | 0.222 |
| 0.265 | |||
| 0ng/ml | 0.05 | 0.055 | 0 |
| 0.061 |
Precision
We measured random samples of E4637Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 10.8 | 0.41 | 3.8 |
| Intra-Assay | 2 | 18 | 3 | 0.19 | 6.2 |
| Intra-Assay | 3 | 18 | 7.1 | 0.44 | 6.2 |
Protocols
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