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Home > Product > ELISA Kit > Mouse Cytotoxic T Lymphocyte Associated Antigen 4, CTLA-4 ELISA Kit

Mouse Cytotoxic T Lymphocyte Associated Antigen 4, CTLA-4 ELISA Kit

Mouse Cytotoxic T Lymphocyte Associated Antigen 4, CTLA-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.058ng/mL

Detection range: 0.1-35ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.058ng/mL

Detection range: 0.1-35ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Cytotoxic T Lymphocyte Associated Antigen 4

Code

E1996Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.058ng/mL

Detection range

0.1-35ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

UniProt accession

MASS(Da)

24,993

GeneID

12477

Synonyms

CD antigen CD152;CTLA 4;CTLA4;CTLA-4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4

Gene names

CTLA4

Research area

Immunology

Target protein

CTLA4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1996Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/mL 2.064 2.21 2.148
2.356
10ng/mL 1.514 1.503 1.441
1.492
5ng/mL 0.795 0.795 0.733
0.795
2.5ng/mL 0.442 0.446 0.383
0.449
1.25ng/mL 0.297 0.299 0.237
0.302
0ng/mL 0.068 0.062 0
0.056

Precision

We measured random samples of E1996Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.8 0.26 5.3
Intra-Assay 2 18 11.3 0.51 4.5
Intra-Assay 3 18 3.3 0.13 4

Protocols

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  • Size

    Code

    Price

  • 48T

    E1996Mo

    $320.00

  • 96T

    E1996Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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