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Home > Product > ELISA Kit > Mouse Dickkopf-related Protein 3, DKK3 ELISA Kit

Mouse Dickkopf-related Protein 3, DKK3 ELISA Kit

Mouse Dickkopf-related Protein 3, DKK3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.35ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 2.35ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Dickkopf-Related Protein 3

Code

E2007Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.35ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

UniProt accession

MASS(Da)

38,388

GeneID

50781

Synonyms

Dickkopf-3;Dickkopf-related protein 3;DKK 3;DKK3;Dkk-3;mDkk-3

Gene names

DKK3

Research area

Stem Cells

Target protein

DKK3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2007Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.23 2.273 2.233
2.317
320ng/L 1.136 1.148 1.107
1.161
160ng/L 0.823 0.824 0.783
0.824
80ng/L 0.466 0.479 0.438
0.491
40ng/L 0.238 0.252 0.211
0.266
0ng/L 0.047 0.041 0
0.035

Precision

We measured random samples of E2007Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 183.3 9.45 5.2
Intra-Assay 2 18 322.9 12.42 3.8
Intra-Assay 3 18 78.5 4.48 5.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E2007Mo

    $320.00

  • 96T

    E2007Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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