Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
10000 pg/ml | 1.673 | 1.659 | 1.551 |
1.646 | |||
5000 pg/ml | 1.024 | 1.012 | 0.904 |
1 | |||
2500 pg/ml | 0.648 | 0.617 | 0.509 |
0.587 | |||
1250 pg/ml | 0.365 | 0.368 | 0.26 |
0.372 | |||
625 pg/ml | 0.234 | 0.238 | 0.13 |
0.243 | |||
312.5 pg/ml | 0.167 | 0.171 | 0.063 |
0.176 | |||
156.25 pg/ml | 0.14 | 0.142 | 0.034 |
0.144 | |||
0 pg/ml | 0.103 | 0.108 | 0 |
0.114 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 632 | 38 | 6 |
Intra-assay | 2 | 20 | 1620 | 57.7 | 3.6 |
Intra-assay | 3 | 20 | 5787 | 253 | 4.4 |
Inter-assay | 1 | 20 | 629 | 59.5 | 9.5 |
Inter-assay | 2 | 20 | 1625 | 79.3 | 4.9 |
Inter-assay | 3 | 20 | 5772 | 265 | 4.6 |
Protocols
References
Customer reviews and Q&As