Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
10000 pg/ml | 1.723 | 1.813 | 1.722 |
1.903 | |||
5000 pg/ml | 1.222 | 1.241 | 1.15 |
1.259 | |||
2500 pg/ml | 0.757 | 0.731 | 0.64 |
0.705 | |||
1250 pg/ml | 0.476 | 0.458 | 0.367 |
0.439 | |||
625 pg/ml | 0.292 | 0.281 | 0.19 |
0.27 | |||
312.5 pg/ml | 0.172 | 0.175 | 0.084 |
0.179 | |||
156.25 pg/ml | 0.126 | 0.132 | 0.041 |
0.138 | |||
0 pg/ml | 0.087 | 0.091 | 0 |
0.095 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 1035 | 69.3 | 6.7 |
Intra-assay | 2 | 20 | 2874 | 134 | 4.7 |
Intra-assay | 3 | 20 | 7768 | 231 | 3 |
Inter-assay | 1 | 20 | 1027 | 75.9 | 7.4 |
Inter-assay | 2 | 20 | 2862 | 143 | 5 |
Inter-assay | 3 | 20 | 7726 | 359 | 4.6 |
Protocols
References
Customer reviews and Q&As