Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
6000 pg/ml | 2.279 | 2.323 | 2.206 |
2.366 | |||
3000 pg/ml | 1.273 | 1.314 | 1.197 |
1.354 | |||
1500 pg/ml | 0.77 | 0.76 | 0.643 |
0.749 | |||
750 pg/ml | 0.417 | 0.436 | 0.319 |
0.454 | |||
375 pg/ml | 0.278 | 0.269 | 0.152 |
0.26 | |||
187.5 pg/ml | 0.183 | 0.185 | 0.068 |
0.186 | |||
93.75 pg/ml | 0.164 | 0.156 | 0.039 |
0.147 | |||
0 pg/ml | 0.109 | 0.117 | 0 |
0.125 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 323 | 25.5 | 7.9 |
Intra-assay | 2 | 20 | 1010 | 49.7 | 4.9 |
Intra-assay | 3 | 20 | 4589 | 154 | 3.4 |
Inter-assay | 1 | 20 | 325 | 28.4 | 8.8 |
Inter-assay | 2 | 20 | 1003 | 69.1 | 6.9 |
Inter-assay | 3 | 20 | 4597 | 274 | 6 |
Protocols
Product publications
Customer reviews and Q&As