Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
5000 pg/ml | 2.714 | 2.793 | 2.702 |
2.872 | |||
2500 pg/ml | 1.404 | 1.415 | 1.324 |
1.426 | |||
1250 pg/ml | 0.815 | 0.836 | 0.745 |
0.857 | |||
625 pg/ml | 0.443 | 0.457 | 0.366 |
0.47 | |||
312.5 pg/ml | 0.256 | 0.275 | 0.184 |
0.294 | |||
156.25 pg/ml | 0.186 | 0.185 | 0.094 |
0.184 | |||
78.125 pg/ml | 0.126 | 0.132 | 0.041 |
0.138 | |||
0 pg/ml | 0.088 | 0.091 | 0 |
0.094 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 590 | 27.5 | 4.7 |
Intra-assay | 2 | 20 | 1686 | 47.7 | 2.8 |
Intra-assay | 3 | 20 | 3440 | 83.1 | 2.4 |
Inter-assay | 1 | 20 | 585 | 38.2 | 6.5 |
Inter-assay | 2 | 20 | 1696 | 81 | 4.8 |
Inter-assay | 3 | 20 | 3467 | 176 | 5.1 |
Protocols
Product publications
Customer reviews and Q&As