Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Mouse E3 Ubiquitin-protein Ligase Smurf1, SMURF1 ELISA Kit

Mouse E3 Ubiquitin-protein Ligase Smurf1, SMURF1 ELISA Kit

Mouse E3 Ubiquitin-protein Ligase Smurf1, SMURF1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.56ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.56ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

E3 Ubiquitin-Protein Ligase SMURF1

Code

E2036Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.56ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway (By similarity). Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed: 23087404). Plays a role in dendrite formation by melanocytes (By similarity).

UniProt accession

MASS(Da)

83,356

GeneID

75788

Synonyms

E3 ubiquitin-protein ligase SMURF1;HECT-type E3 ubiquitin transferase SMURF1;SMAD ubiquitination regulatory factor 1;SMAD-specific E3 ubiquitin-protein ligase 1;SMURF 1;SMURF1

Gene names

SMURF1

Research area

Cell Biology

Target protein

SMURF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2036Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.08 2.066 2.007
2.051
1200ng/L 1.095 0.996 0.938
0.898
600ng/L 0.556 0.57 0.511
0.584
300ng/L 0.212 0.237 0.178
0.262
150ng/L 0.12 0.139 0.08
0.158
0ng/L 0.056 0.059 0
0.061

Precision

We measured random samples of E2036Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1861 31 1.7
Intra-Assay 2 18 708 41.16 5.8
Intra-Assay 3 18 1412.2 67.27 4.8

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E2036Mo

    $320.00

  • 96T

    E2036Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up