Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
3200 pg/ml | 2.387 | 2.31 | 2.207 |
2.233 | |||
1600 pg/ml | 1.335 | 1.269 | 1.166 |
1.204 | |||
800 pg/ml | 0.749 | 0.702 | 0.599 |
0.656 | |||
400 pg/ml | 0.38 | 0.381 | 0.278 |
0.383 | |||
200 pg/ml | 0.238 | 0.24 | 0.137 |
0.243 | |||
100 pg/ml | 0.18 | 0.168 | 0.065 |
0.157 | |||
50 pg/ml | 0.139 | 0.134 | 0.031 |
0.13 | |||
0 pg/ml | 0.106 | 0.103 | 0 |
0.101 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 333 | 22.6 | 6.8 |
Intra-assay | 2 | 20 | 679 | 24.1 | 3.5 |
Intra-assay | 3 | 20 | 1997 | 96.8 | 4.8 |
Inter-assay | 1 | 20 | 335 | 20.5 | 6.1 |
Inter-assay | 2 | 20 | 671 | 33.6 | 5 |
Inter-assay | 3 | 20 | 1990 | 110 | 5.5 |
Protocols
References
Customer reviews and Q&As