Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
3000 pg/ml | 2.656 | 2.688 | 2.582 |
2.72 | |||
1500 pg/ml | 1.481 | 1.461 | 1.355 |
1.441 | |||
750 pg/ml | 0.826 | 0.793 | 0.687 |
0.76 | |||
375 pg/ml | 0.488 | 0.462 | 0.356 |
0.436 | |||
187.5 pg/ml | 0.293 | 0.287 | 0.181 |
0.281 | |||
93.75 pg/ml | 0.183 | 0.191 | 0.085 |
0.199 | |||
46.875 pg/ml | 0.142 | 0.144 | 0.038 |
0.146 | |||
0 pg/ml | 0.113 | 0.106 | 0 |
0.099 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 144 | 7.7 | 5.3 |
Intra-assay | 2 | 20 | 496 | 17.7 | 3.6 |
Intra-assay | 3 | 20 | 1838 | 54.7 | 3 |
Inter-assay | 1 | 20 | 146 | 12.6 | 8.7 |
Inter-assay | 2 | 20 | 493 | 48.4 | 9.8 |
Inter-assay | 3 | 20 | 1824 | 181 | 9.9 |
Protocols
References
Customer reviews and Q&As