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Home > Product > ELISA Kit > Mouse Ephrin-A1, EFNA1 ELISA Kit

Mouse Ephrin-A1, EFNA1 ELISA Kit

Mouse Ephrin-A1, EFNA1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 23.43ng/L

Detection range: 50-10000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 23.43ng/L

Detection range: 50-10000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Ephrin-A1

Code

E2060Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

23.43ng/L

Detection range

50-10000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.

UniProt accession

MASS(Da)

23,802

GeneID

13636

Synonyms

EFNA 1;EFNA1;EPH-related receptor tyrosine kinase ligand 1;Ephrin-A1;Ephrin-A1, secreted form;Immediate early response protein B61;LERK-1

Gene names

EFNA1

Research area

Cardiovascular

Target protein

EFNA1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2060Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6400ng/L 2.138 1.987 1.95
1.837
3200ng/L 1.002 1.003 0.965
1.003
1600ng/L 0.432 0.47 0.433
0.509
800ng/L 0.191 0.201 0.164
0.211
400ng/L 0.089 0.103 0.066
0.117
0ng/L 0.034 0.037 0
0.041

Precision

We measured random samples of E2060Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4110.5 104.01 2.5
Intra-Assay 2 18 3287 118.14 3.6
Intra-Assay 3 18 1315 47.45 3.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E2060Mo

    $320.00

  • 96T

    E2060Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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