Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
2000 pg/ml | 2.048 | 2.14 | 2.056 |
2.233 | |||
1000 pg/ml | 1.089 | 1.13 | 1.046 |
1.171 | |||
500 pg/ml | 0.64 | 0.61 | 0.526 |
0.581 | |||
250 pg/ml | 0.353 | 0.349 | 0.265 |
0.346 | |||
125 pg/ml | 0.206 | 0.217 | 0.133 |
0.229 | |||
62.5 pg/ml | 0.153 | 0.15 | 0.066 |
0.148 | |||
31.25 pg/ml | 0.122 | 0.114 | 0.03 |
0.107 | |||
0 pg/ml | 0.084 | 0.084 | 0 |
0.085 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 68.3 | 3.7 | 5.4 |
Intra-assay | 2 | 20 | 342 | 14.4 | 4.2 |
Intra-assay | 3 | 20 | 1424 | 92.9 | 6.5 |
Inter-assay | 1 | 20 | 68.6 | 7.5 | 11 |
Inter-assay | 2 | 20 | 344 | 27.7 | 8.1 |
Inter-assay | 3 | 20 | 1419 | 120 | 8.5 |
Protocols
References
Customer reviews and Q&As