Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
2000 pg/ml | 2.389 | 2.484 | 2.389 |
2.58 | |||
1000 pg/ml | 1.404 | 1.36 | 1.265 |
1.316 | |||
500 pg/ml | 0.74 | 0.789 | 0.694 |
0.839 | |||
250 pg/ml | 0.469 | 0.445 | 0.35 |
0.422 | |||
125 pg/ml | 0.275 | 0.269 | 0.174 |
0.264 | |||
62.5 pg/ml | 0.176 | 0.185 | 0.09 |
0.194 | |||
31.25 pg/ml | 0.14 | 0.138 | 0.043 |
0.137 | |||
0 pg/ml | 0.099 | 0.095 | 0 |
0.091 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 317 | 11.7 | 3.7 |
Intra-assay | 2 | 20 | 738 | 23.6 | 3.2 |
Intra-assay | 3 | 20 | 1570 | 45.9 | 2.9 |
Inter-assay | 1 | 20 | 317 | 26.1 | 8.2 |
Inter-assay | 2 | 20 | 743 | 60.3 | 8.1 |
Inter-assay | 3 | 20 | 1578 | 77.1 | 4.9 |
Protocols
References
Customer reviews and Q&As