Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1500 pg/ml | 1.98 | 2.075 | 1.972 |
2.17 | |||
750 pg/ml | 1.229 | 1.168 | 1.065 |
1.107 | |||
375 pg/ml | 0.651 | 0.664 | 0.561 |
0.677 | |||
187.5 pg/ml | 0.401 | 0.394 | 0.291 |
0.386 | |||
93.75 pg/ml | 0.248 | 0.257 | 0.154 |
0.265 | |||
46.875 pg/ml | 0.184 | 0.177 | 0.074 |
0.169 | |||
23.4375 pg/ml | 0.153 | 0.144 | 0.041 |
0.135 | |||
0 pg/ml | 0.105 | 0.103 | 0 |
0.101 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 111 | 10.2 | 9.2 |
Intra-assay | 2 | 20 | 277 | 27.4 | 9.9 |
Intra-assay | 3 | 20 | 990 | 96 | 9.7 |
Inter-assay | 1 | 20 | 112 | 12.6 | 11.2 |
Inter-assay | 2 | 20 | 279 | 29.6 | 10.6 |
Inter-assay | 3 | 20 | 1000 | 91.6 | 9.2 |
Protocols
References
Customer reviews and Q&As