Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1000 pg/ml | 2.528 | 2.551 | 2.464 |
2.574 | |||
500 pg/ml | 1.354 | 1.372 | 1.285 |
1.39 | |||
250 pg/ml | 0.694 | 0.735 | 0.648 |
0.776 | |||
125 pg/ml | 0.419 | 0.398 | 0.311 |
0.377 | |||
62.5 pg/ml | 0.234 | 0.24 | 0.153 |
0.245 | |||
31.25 pg/ml | 0.158 | 0.166 | 0.079 |
0.174 | |||
15.625 pg/ml | 0.13 | 0.124 | 0.037 |
0.118 | |||
0 pg/ml | 0.086 | 0.087 | 0 |
0.087 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 109 | 5.2 | 4.8 |
Intra-assay | 2 | 20 | 259 | 12.6 | 4.8 |
Intra-assay | 3 | 20 | 550 | 23.6 | 4.3 |
Inter-assay | 1 | 20 | 110 | 7.3 | 6.7 |
Inter-assay | 2 | 20 | 262 | 16.6 | 6.3 |
Inter-assay | 3 | 20 | 546 | 33.8 | 6.2 |
Protocols
References
Customer reviews and Q&As