Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
500 pg/ml | 1.886 | 1.988 | 1.893 |
2.091 | |||
250 pg/ml | 1.165 | 1.177 | 1.082 |
1.19 | |||
125 pg/ml | 0.68 | 0.681 | 0.586 |
0.682 | |||
62.5 pg/ml | 0.402 | 0.396 | 0.301 |
0.39 | |||
31.25 pg/ml | 0.259 | 0.257 | 0.162 |
0.255 | |||
15.625 pg/ml | 0.171 | 0.174 | 0.079 |
0.177 | |||
7.8125 pg/ml | 0.135 | 0.135 | 0.04 |
0.136 | |||
0 pg/ml | 0.094 | 0.095 | 0 |
0.097 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 61.7 | 2.8 | 4.5 |
Intra-assay | 2 | 20 | 144 | 6.3 | 4.3 |
Intra-assay | 3 | 20 | 359 | 19.3 | 5.4 |
Inter-assay | 1 | 20 | 61 | 2.6 | 4.3 |
Inter-assay | 2 | 20 | 143 | 6.1 | 4.3 |
Inter-assay | 3 | 20 | 363 | 18.8 | 5.2 |
Protocols
References
Customer reviews and Q&As