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Home > Product > ELISA Kit > Mouse Gamma-Glutamyltranspeptidase 1, GGT1 ELISA Kit

Mouse Gamma-Glutamyltranspeptidase 1, GGT1 ELISA Kit

Mouse Gamma-Glutamyltranspeptidase 1, GGT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.112mU/mL

Detection range: 0.2-60mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.112mU/mL

Detection range: 0.2-60mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Gamma-Glutamyltranspeptidase 1

Code

E2090Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.112mU/mL

Detection range

0.2-60mU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4 (By similarity). Indirectly regulates multiple aspects of skeletal biology (PubMed: 12810527).

UniProt accession

MASS(Da)

61,563

GeneID

14598

Synonyms

CD antigen CD224;Gamma-glutamyltransferase 1;Gamma-glutamyltranspeptidase 1;GGT 1;GGT1;Glutathione hydrolase 1 heavy chain;Glutathione hydrolase 1 light chain;Glutathione hydrolase 1 proenzyme;Leukotriene-C4 hydrolase;

Gene names

GGT1

Research area

Signal Transduction

Target protein

GGT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2090Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32mU/mL 2.086 2.103 2.046
2.119
16mU/mL 1.734 1.719 1.662
1.703
8mU/mL 1.181 1.154 1.098
1.128
4mU/mL 0.636 0.639 0.582
0.642
2mU/mL 0.394 0.395 0.339
0.397
0mU/mL 0.052 0.057 0
0.061

Precision

We measured random samples of E2090Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.9 0.15 5.3
Intra-Assay 2 18 11.1 0.5 4.5
Intra-Assay 3 18 5.2 0.27 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E2090Mo

    $320.00

  • 96T

    E2090Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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