Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
300 pg/ml | 2.237 | 2.24 | 2.115 |
2.243 | |||
150 pg/ml | 1.36 | 1.339 | 1.214 |
1.318 | |||
75 pg/ml | 0.708 | 0.735 | 0.61 |
0.762 | |||
37.5 pg/ml | 0.436 | 0.439 | 0.314 |
0.442 | |||
18.75 pg/ml | 0.271 | 0.278 | 0.153 |
0.286 | |||
9.375 pg/ml | 0.207 | 0.202 | 0.077 |
0.198 | |||
4.6875 pg/ml | 0.164 | 0.165 | 0.04 |
0.167 | |||
0 pg/ml | 0.117 | 0.125 | 0 |
0.134 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 21.5 | 1 | 4.6 |
Intra-assay | 2 | 20 | 51.1 | 1 | 2 |
Intra-assay | 3 | 20 | 189 | 4.3 | 2.3 |
Inter-assay | 1 | 20 | 21.7 | 1.5 | 6.8 |
Inter-assay | 2 | 20 | 51.3 | 1.9 | 3.7 |
Inter-assay | 3 | 20 | 188 | 8 | 4.3 |
Protocols
References
Customer reviews and Q&As