Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
75 pg/ml | 2.565 | 2.599 | 2.465 |
2.633 | |||
37.5 pg/ml | 1.262 | 1.331 | 1.197 |
1.401 | |||
18.75 pg/ml | 0.765 | 0.741 | 0.607 |
0.718 | |||
9.375 pg/ml | 0.432 | 0.435 | 0.301 |
0.439 | |||
4.6875 pg/ml | 0.285 | 0.283 | 0.149 |
0.281 | |||
2.34375 pg/ml | 0.189 | 0.199 | 0.065 |
0.21 | |||
1.171875 pg/ml | 0.166 | 0.163 | 0.029 |
0.161 | |||
0 pg/ml | 0.14 | 0.134 | 0 |
0.129 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 5.6 | 0.18 | 3.3 |
Intra-assay | 2 | 20 | 17.1 | 0.49 | 2.9 |
Intra-assay | 3 | 20 | 40.7 | 0.92 | 2.3 |
Inter-assay | 1 | 20 | 5.6 | 0.36 | 6.4 |
Inter-assay | 2 | 20 | 17.1 | 0.8 | 4.7 |
Inter-assay | 3 | 20 | 40.4 | 1.9 | 4.8 |
Protocols
References
Customer reviews and Q&As