Product overview
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Background
Human CD63 antigen, also referred to as Granulophysin, Lysomal-associated membrane protein 3 (LAMP-3), Tetraspanin-30, and Ocular melanoma-associated antigen, is a membrane bound protein encoded by the gene CD63. CD63 belongs to a group of proteins known as the tetraspanin family, which are characterized by the presence of four hydrophobic domains and are found to mediate signal transduction events for the regulation of cell development, activation, growth, and motility. Deficiency of this protein is associated with Hermansky-Pudlak Syndrome, which is an autosomal disorder.
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Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5055Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 48ng/ml | 2.094 | 2.167 | 2.101 |
| 2.24 | |||
| 24ng/ml | 1.427 | 1.378 | 1.312 |
| 1.329 | |||
| 12ng/ml | 0.843 | 0.846 | 0.781 |
| 0.85 | |||
| 6ng/ml | 0.53 | 0.491 | 0.426 |
| 0.453 | |||
| 3ng/ml | 0.249 | 0.241 | 0.175 |
| 0.233 | |||
| 0ng/ml | 0.057 | 0.066 | 0 |
| 0.074 |
Precision
We measured random samples of E5055Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 25.238 | 0.917 | 3.6 |
| Intra-assay | 2 | 18 | 14.376 | 0.726 | 5 |
| Intra-assay | 3 | 18 | 10.181 | 0.528 | 5.2 |
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