Product overview
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Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1393Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 160ng/L | 2.161 | 2.124 | 2.098 |
| 2.086 | |||
| 80ng/L | 1.399 | 1.328 | 1.303 |
| 1.258 | |||
| 40ng/L | 0.63 | 0.675 | 0.65 |
| 0.72 | |||
| 20ng/L | 0.392 | 0.412 | 0.386 |
| 0.431 | |||
| 10ng/L | 0.119 | 0.15 | 0.125 |
| 0.182 | |||
| 0ng/L | 0.033 | 0.026 | 0 |
| 0.018 |
Precision
We measured random samples of E1393Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 51.6 | 2.3 | 4.4 |
| Intra-assay | 2 | 18 | 93.3 | 3.6 | 3.9 |
| Intra-assay | 3 | 18 | 34.8 | 1.7 | 4.8 |
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