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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1981Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.122	2.198	2.148
	2.275
40ng/ml	1.424	1.419	1.369
	1.415
20ng/ml	0.553	0.618	0.567
	0.683
10ng/ml	0.379	0.398	0.347
	0.416
5ng/ml	0.188	0.177	0.126
	0.166
0ng/ml	0.042	0.051	0
	0.059

We measured random samples of E1981Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	25.4	1.1	4.1
Intra-assay	2	18	55	2.3	4.2
Intra-assay	3	18	16.1	0.7	4.4

> Protocol Booklet

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