Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1425Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200nmol/L | 2.032 | 2.167 | 2.122 |
| 2.302 | |||
| 600nmol/L | 1.325 | 1.376 | 1.331 |
| 1.426 | |||
| 300nmol/L | 0.603 | 0.683 | 0.638 |
| 0.764 | |||
| 150nmol/L | 0.419 | 0.391 | 0.346 |
| 0.364 | |||
| 75nmol/L | 0.184 | 0.17 | 0.125 |
| 0.156 | |||
| 0nmol/L | 0.05 | 0.045 | 0 |
| 0.04 |
Precision
We measured random samples of E1425Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 148.8 | 5.6 | 3.8 |
| Intra-assay | 2 | 18 | 759.7 | 26.5 | 3.5 |
| Intra-assay | 3 | 18 | 529.9 | 24.5 | 4.6 |
Protocols
References
Customer reviews and Q&As
