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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1436Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.115	2.183	2.111
	2.25
32ng/ml	1.193	1.157	1.085
	1.121
16ng/ml	0.567	0.578	0.506
	0.589
8ng/ml	0.298	0.282	0.21
	0.267
4ng/ml	0.157	0.15	0.078
	0.143
0ng/ml	0.069	0.072	0
	0.075

We measured random samples of E1436Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	22.9	0.7	3.1
Intra-assay	2	18	37.7	1.1	2.9
Intra-assay	3	18	12.4	0.6	4.6

> Protocol Booklet

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