Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1436Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 64ng/ml | 2.115 | 2.183 | 2.111 |
| 2.25 | |||
| 32ng/ml | 1.193 | 1.157 | 1.085 |
| 1.121 | |||
| 16ng/ml | 0.567 | 0.578 | 0.506 |
| 0.589 | |||
| 8ng/ml | 0.298 | 0.282 | 0.21 |
| 0.267 | |||
| 4ng/ml | 0.157 | 0.15 | 0.078 |
| 0.143 | |||
| 0ng/ml | 0.069 | 0.072 | 0 |
| 0.075 |
Precision
We measured random samples of E1436Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 22.9 | 0.7 | 3.1 |
| Intra-assay | 2 | 18 | 37.7 | 1.1 | 2.9 |
| Intra-assay | 3 | 18 | 12.4 | 0.6 | 4.6 |
Protocols
References
Customer reviews and Q&As
