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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0758Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32mg/L	2.417	2.398	2.357
	2.38
16mg/L	1.218	1.261	1.22
	1.305
8mg/L	0.712	0.647	0.606
	0.582
4mg/L	0.373	0.347	0.305
	0.321
2mg/L	0.133	0.159	0.117
	0.185
0mg/L	0.037	0.041	0
	0.046

We measured random samples of E0758Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	12.3	0.7	5.3
Intra-assay	2	18	20.3	0.5	2.3
Intra-assay	3	18	5.4	0.3	4.8

> Protocol Booklet

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