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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1887Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40000ng/L	2.196	2.238	2.174
	2.28
20000ng/L	1.481	1.401	1.337
	1.322
10000ng/L	0.912	0.871	0.807
	0.831
5000ng/L	0.53	0.477	0.413
	0.425
2500ng/L	0.218	0.22	0.156
	0.223
0ng/L	0.062	0.064	0
	0.066

We measured random samples of E1887Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	17679.2	620.4	3.5
Intra-assay	2	18	6717.7	401.8	6
Intra-assay	3	18	14801.1	796	5.4

> Protocol Booklet

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