Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E7379Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
120ng/ml | 1.985 | 2.021 | 1.939 |
2.058 | |||
60ng/ml | 1.071 | 1.101 | 1.019 |
1.131 | |||
30ng/ml | 0.552 | 0.542 | 0.46 |
0.532 | |||
15ng/ml | 0.317 | 0.324 | 0.242 |
0.33 | |||
7.5ng/ml | 0.16 | 0.169 | 0.087 |
0.179 | |||
0ng/ml | 0.086 | 0.082 | 0 |
0.077 |
Precision
We measured random samples of E7379Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 106.4 | 3.2 | 3 |
Intra-assay | 2 | 18 | 58.9 | 2.9 | 5 |
Intra-assay | 3 | 18 | 31.4 | 1.7 | 5.5 |
Protocols
References
Customer reviews and Q&As