Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1911Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
240ng/ml | 2.297 | 2.345 | 2.286 |
2.393 | |||
120ng/ml | 1.302 | 1.327 | 1.268 |
1.352 | |||
60ng/ml | 0.878 | 0.779 | 0.72 |
0.68 | |||
30ng/ml | 0.456 | 0.463 | 0.404 |
0.469 | |||
15ng/ml | 0.257 | 0.289 | 0.231 |
0.322 | |||
0ng/ml | 0.054 | 0.059 | 0 |
0.064 |
Precision
We measured random samples of E1911Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 140 | 4.2 | 3 |
Intra-assay | 2 | 18 | 100.9 | 6.1 | 6.1 |
Intra-assay | 3 | 18 | 27.2 | 1.8 | 6.8 |
Protocols
References
Customer reviews and Q&As