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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0174Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.389	2.386	2.324
	2.383
60ng/ml	1.51	1.436	1.375
	1.363
30ng/ml	0.941	0.828	0.767
	0.716
15ng/ml	0.382	0.394	0.333
	0.406
7.5ng/ml	0.189	0.192	0.13
	0.195
0ng/ml	0.056	0.061	0
	0.066

We measured random samples of E0174Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	35.6	1.5	4.2
Intra-assay	2	18	58.9	2	3.4
Intra-assay	3	18	27	1.2	4.3

> Protocol Booklet

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