Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0019Fi are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1200mU/ml | 2.034 | 1.978 | 1.896 |
1.923 | |||
600mU/ml | 1.052 | 1.092 | 1.009 |
1.131 | |||
300mU/ml | 0.595 | 0.615 | 0.532 |
0.634 | |||
150mU/ml | 0.298 | 0.311 | 0.228 |
0.324 | |||
75mU/ml | 0.166 | 0.155 | 0.073 |
0.145 | |||
0mU/ml | 0.085 | 0.083 | 0 |
0.08 |
Precision
We measured random samples of E0019Fi within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 474.2 | 21 | 4.4 |
Intra-assay | 2 | 18 | 784.2 | 22.3 | 2.8 |
Intra-assay | 3 | 18 | 223.6 | 11.5 | 5.2 |
Protocols
References
Customer reviews and Q&As