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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1061Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.275	2.254	2.21
	2.233
160ng/L	1.45	1.485	1.441
	1.52
80ng/L	0.894	0.862	0.818
	0.83
40ng/L	0.482	0.49	0.446
	0.499
20ng/L	0.276	0.272	0.228
	0.267
0ng/L	0.051	0.044	0
	0.037

We measured random samples of E1061Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	153.5	5.6	3.6
Intra-assay	2	18	68.9	3.3	4.8
Intra-assay	3	18	55.1	3.4	6.2

> Protocol Booklet

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