Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1061Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 320ng/L | 2.275 | 2.254 | 2.21 |
| 2.233 | |||
| 160ng/L | 1.45 | 1.485 | 1.441 |
| 1.52 | |||
| 80ng/L | 0.894 | 0.862 | 0.818 |
| 0.83 | |||
| 40ng/L | 0.482 | 0.49 | 0.446 |
| 0.499 | |||
| 20ng/L | 0.276 | 0.272 | 0.228 |
| 0.267 | |||
| 0ng/L | 0.051 | 0.044 | 0 |
| 0.037 |
Precision
We measured random samples of E1061Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 153.5 | 5.6 | 3.6 |
| Intra-assay | 2 | 18 | 68.9 | 3.3 | 4.8 |
| Intra-assay | 3 | 18 | 55.1 | 3.4 | 6.2 |
Protocols
References
Customer reviews and Q&As
