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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1129Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.259	2.353	2.293
	2.447
6ng/ml	1.299	1.389	1.329
	1.479
3ng/ml	0.877	0.817	0.758
	0.757
1.5ng/ml	0.529	0.492	0.432
	0.455
0.75ng/ml	0.278	0.257	0.197
	0.236
0ng/ml	0.042	0.06	0
	0.078

We measured random samples of E1129Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	1.3	0.1	5.1
Intra-assay	2	18	4.1	0.2	6
Intra-assay	3	18	6.5	0.2	3

> Protocol Booklet

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