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Home > Product > ELISA Kit > Rat Vitamin D-binding protein, DBP ELISA Kit

Rat Vitamin D-binding protein, DBP ELISA Kit

Rat Vitamin D-binding protein, DBP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.12ug/ml

Detection range: 2-600ug/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 1.12ug/ml

Detection range: 2-600ug/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Rat

Product overview

Full product name

Vitamin D-binding protein

Code

E0616Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.12ug/ml

Detection range

2-600ug/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Rat

Storage

2-8°C

Assay time

1h 30m

Background

Vitamin D Binding Protein, also known as gc-globulin, is a 458 - amino acid, glycosylated protein encoded by the gene GC, which is in the albumin gene family. The primary function of this protein is to bind Vitamin D and its metabolites and to transport them to tissue. Vitamin D Binding protein is highly expressed and secreted into serum primarily from hepatic cells. There are three common alleles of the protein (Gc1f, Gc1s, and Gc2), and several minor polymorphisms. Different glycosylation sites are found on the isoforms of Vitamin D binding protein, a selectively deglycosylated form produced by B cell ß-galactosidase and T cell sialidase is known as Macrophage Activating Factor. MAF has been found to block angiogenic effects of several proteins on vascular endothelial cells.

UniProt accession

MASS(Da)

53,544

Synonyms

Gc;Dbp

Gene names

Gc

Research area

Signal Transduction|Cancer|Metabolism

Target protein

DBP

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E0616Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ug/ml 2.023 1.965 1.88
1.906
200ug/ml 1.146 1.17 1.086
1.195
100ug/ml 0.581 0.569 0.484
0.557
50ug/ml 0.284 0.273 0.188
0.261
25ug/ml 0.16 0.17 0.085
0.18
0ug/ml 0.088 0.085 0
0.081

Precision

We measured random samples of E0616Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 236.4 8.5 3.6
Intra-assay 2 18 75.7 3.3 4.4
Intra-assay 3 18 141.9 6.1 4.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0616Ra

    $320.00

  • 96T

    E0616Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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