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Home > Product > ELISA Kit > Human Dickkopf-3, DKK3 ELISA Kit

Human Dickkopf-3, DKK3 ELISA Kit

Human Dickkopf-3, DKK3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.06ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.06ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Dickkopf-3

Code

E2065Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.06ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

UniProt accession

MASS(Da)

38,390

GeneID

27122

Synonyms

DKK3;REIC;UNQ258;PRO295

Gene names

DKK3

Research area

Signal Transduction;Stem Cells

Target protein

DKK3

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E2065Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.198 2.136 2.097
2.073
12ng/ml 1.314 1.368 1.329
1.422
6ng/ml 0.642 0.679 0.64
0.715
3ng/ml 0.45 0.388 0.349
0.326
1.5ng/ml 0.193 0.164 0.125
0.135
0ng/ml 0.035 0.039 0
0.043

Precision

We measured random samples of E2065Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 3.5 0.1 3.8
Intra-assay 2 18 12 0.5 3.9
Intra-assay 3 18 8.6 0.5 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E2065Hu

    $320.00

  • 96T

    E2065Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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