Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1455Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
120ng/ml | 2.175 | 2.189 | 2.12 |
2.203 | |||
60ng/ml | 1.175 | 1.144 | 1.075 |
1.113 | |||
30ng/ml | 0.568 | 0.582 | 0.513 |
0.596 | |||
15ng/ml | 0.319 | 0.336 | 0.267 |
0.352 | |||
7.5ng/ml | 0.167 | 0.163 | 0.094 |
0.159 | |||
0ng/ml | 0.064 | 0.069 | 0 |
0.073 |
Precision
We measured random samples of E1455Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 63.8 | 3.3 | 5.2 |
Intra-assay | 2 | 18 | 82 | 2 | 2.5 |
Intra-assay | 3 | 18 | 34.4 | 1.5 | 4.4 |
Protocols
References
Customer reviews and Q&As