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Home > Product > ELISA Kit > Rabbit Epidermal Growth Factor, EGF ELISA Kit

Rabbit Epidermal Growth Factor, EGF ELISA Kit

Rabbit Epidermal Growth Factor, EGF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.12ng/L

Detection range: 10-3000ng/L

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Rabbit

Product Summary

Size: 48T,96T

Sensitivity: 5.12ng/L

Detection range: 10-3000ng/L

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Rabbit

Product overview

Full product name

Epidermal growth factor

Code

E0023Rb

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.12ng/L

Detection range

10-3000ng/L

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Rabbit

Storage

2-8°C

Assay time

1h 30m

Background

EGF precursor is a large 1185 amino acid group 1 member of the EGF family growth factors. All EGF family growth factors are synthesized as transmembrane proteins that undergo proteolytic processing to generate soluble forms. Human EGF is a 53 amino acid long fragment of the extracellular domain of EGF precursor. As other group 1 members, EGF binds to, and activates the EGF receptor. This promotes EGFR dimerization. EGF has variety of diverse physiological effects. EGF activity is complicated by the fact that other EGF family members utilize EGF receptor. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.

Research area

Signal Transduction|Cell Biology|Cancer|Neuroscience

Target protein

EGF

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E0023Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.107 2.095 2.015
2.083
800ng/L 1.148 1.127 1.047
1.107
400ng/L 0.575 0.584 0.503
0.593
200ng/L 0.318 0.324 0.243
0.331
100ng/L 0.179 0.174 0.094
0.17
0ng/L 0.088 0.081 0
0.073

Precision

We measured random samples of E0023Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 1168.9 35.7 3.1
Intra-assay 2 18 733.9 34 4.6
Intra-assay 3 18 515 23.5 4.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0023Rb

    $320.00

  • 96T

    E0023Rb

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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