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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0023Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.107	2.095	2.015
	2.083
800ng/L	1.148	1.127	1.047
	1.107
400ng/L	0.575	0.584	0.503
	0.593
200ng/L	0.318	0.324	0.243
	0.331
100ng/L	0.179	0.174	0.094
	0.17
0ng/L	0.088	0.081	0
	0.073

We measured random samples of E0023Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	1168.9	35.7	3.1
Intra-assay	2	18	733.9	34	4.6
Intra-assay	3	18	515	23.5	4.6

> Protocol Booklet

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