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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0102Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800nmol/L	2.128	2.195	2.149
	2.262
400nmol/L	1.538	1.533	1.487
	1.528
200nmol/L	0.571	0.562	0.516
	0.554
100nmol/L	0.358	0.355	0.309
	0.351
50nmol/L	0.139	0.137	0.091
	0.136
0nmol/L	0.045	0.046	0
	0.047

We measured random samples of E0102Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	330.8	15.3	4.6
Intra-assay	2	18	333.4	6.5	1.9
Intra-assay	3	18	160.7	5.6	3.5

> Protocol Booklet

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