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Home > Product > ELISA Kit > Human Glucokinase, GCK ELISA Kit

Human Glucokinase, GCK ELISA Kit

Human Glucokinase, GCK ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.85ng/L

Detection range: 10-2000ng/L

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.85ng/L

Detection range: 10-2000ng/L

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Glucokinase

Code

E0773Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.85ng/L

Detection range

10-2000ng/L

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.

UniProt accession

MASS(Da)

52,191

GeneID

2645

Synonyms

GCK

Gene names

GCK

Research area

Signal Transduction;Cancer;Metabolism

Target protein

GCK

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E0773Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.179 2.208 2.162
2.237
600ng/L 1.181 1.198 1.152
1.216
300ng/L 0.821 0.821 0.775
0.821
150ng/L 0.504 0.514 0.467
0.523
75ng/L 0.26 0.227 0.181
0.194
0ng/L 0.063 0.046 0
0.029

Precision

We measured random samples of E0773Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 263.3 19.3 7.3
Intra-assay 2 18 659.9 23.6 3.6
Intra-assay 3 18 426.7 24.1 5.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E0773Hu

    $320.00

  • 96T

    E0773Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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