Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0023Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
120ng/ml | 2.037 | 2.055 | 1.979 |
2.074 | |||
60ng/ml | 1.052 | 1.037 | 0.96 |
1.021 | |||
30ng/ml | 0.589 | 0.602 | 0.525 |
0.614 | |||
15ng/ml | 0.325 | 0.345 | 0.268 |
0.364 | |||
7.5ng/ml | 0.164 | 0.16 | 0.083 |
0.155 | |||
0ng/ml | 0.071 | 0.077 | 0 |
0.082 |
Precision
We measured random samples of E0023Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 106.9 | 3.4 | 3.2 |
Intra-assay | 2 | 18 | 46 | 2 | 4.5 |
Intra-assay | 3 | 18 | 34.6 | 2.3 | 6.7 |
Protocols
Customer reviews and Q&As