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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0175Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.113	2.133	2.052
	2.153
40ng/ml	1.222	1.202	1.121
	1.182
20ng/ml	0.586	0.61	0.529
	0.633
10ng/ml	0.316	0.299	0.218
	0.281
5ng/ml	0.171	0.166	0.085
	0.161
0ng/ml	0.077	0.081	0
	0.084

We measured random samples of E0175Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	29.3	1.5	5
Intra-assay	2	18	44.1	1.4	3.1
Intra-assay	3	18	13.2	0.5	4.1

> Protocol Booklet

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