Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0108Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 64ng/ml | 2.112 | 2.134 | 2.058 |
| 2.157 | |||
| 32ng/ml | 1.128 | 1.162 | 1.085 |
| 1.195 | |||
| 16ng/ml | 0.545 | 0.525 | 0.449 |
| 0.505 | |||
| 8ng/ml | 0.286 | 0.302 | 0.226 |
| 0.318 | |||
| 4ng/ml | 0.171 | 0.159 | 0.082 |
| 0.147 | |||
| 0ng/ml | 0.07 | 0.076 | 0 |
| 0.082 |
Precision
We measured random samples of E0108Rb within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 48 | 1.7 | 3.5 |
| Intra-assay | 2 | 18 | 21.1 | 0.9 | 4.1 |
| Intra-assay | 3 | 18 | 32.6 | 1.5 | 4.6 |
Protocols
References
Customer reviews and Q&As
