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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0108Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.112	2.134	2.058
	2.157
32ng/ml	1.128	1.162	1.085
	1.195
16ng/ml	0.545	0.525	0.449
	0.505
8ng/ml	0.286	0.302	0.226
	0.318
4ng/ml	0.171	0.159	0.082
	0.147
0ng/ml	0.07	0.076	0
	0.082

We measured random samples of E0108Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	48	1.7	3.5
Intra-assay	2	18	21.1	0.9	4.1
Intra-assay	3	18	32.6	1.5	4.6

> Protocol Booklet

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