Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E3624Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
64ng/ml | 1.97 | 2.011 | 1.989 |
2.052 | |||
32ng/ml | 1.383 | 1.389 | 1.367 |
1.395 | |||
16ng/ml | 0.704 | 0.674 | 0.652 |
0.644 | |||
8ng/ml | 0.304 | 0.341 | 0.318 |
0.377 | |||
4ng/ml | 0.21 | 0.197 | 0.174 |
0.184 | |||
0ng/ml | 0.019 | 0.022 | 0 |
0.025 |
Precision
We measured random samples of E3624Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 27.7 | 1.4 | 5.2 |
Intra-assay | 2 | 18 | 28.9 | 0.9 | 3.1 |
Intra-assay | 3 | 18 | 13 | 0.7 | 5.1 |
Protocols
References
Customer reviews and Q&As