Product overview
Full product name
Size
Sensitivity
Detection range
Sample type
Code
Assay type
Species
Storage
Background
Glutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione is involved in detoxification, it binds to toxins, such as heavy metals, solvents, and pesticides, and transforms them into a form that can be excreted in urine or bile. It is also an important antioxidant, helping to maintain the -SH groups of proteins in their reduced form. Chronic functional glutathione deficiency is associated with glucose 6-phosphate dehydrogenase deficiency, immune disorders, an increased incidence of malignancies, and in the case of HIV disease, probably accelerated pathogenesis of the disease. Acute manifestations of functional glutathione deficiency can be seen in those who have taken an overdosage of acetaminophen (paracetamol). This results in depletion of glutathione in the hepatocytes, leading to liver failure and death.
Research area
Target protein
Components
Components | Quantity |
---|---|
Pre-coated Plate | 12 * 8 well strips x 1 |
Standard, lyophilized | 2 vials |
Standard/Sample Diluent | 6ml ×1 vial |
Biotinylated Antigen, lyophilized | 1 vial |
Avidin-HRP Concentrate | 100μl × 1 vial |
Biotinylated Antigen Diluent | 6ml × 1 vials |
Avidin HRP Diluent | 5.9ml × 1 vials |
Substrate Solution A | 6ml ×1 vial |
Substrate Solution B | 6ml ×1 vial |
Stop Solution | 6ml ×1 vial |
Wash Buffer Concentrate (25x) | 20ml × 1 vial |
Protocol data
These standard curves of EA0113Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
0mg/L | 1.968 | 1.906 | 1.873 |
1.845 | |||
62.5mg/L | 1.575 | 1.536 | 1.503 |
1.498 | |||
125mg/L | 1.179 | 1.199 | 1.166 |
1.219 | |||
250mg/L | 0.71 | 0.68 | 0.647 |
0.65 | |||
500mg/L | 0.365 | 0.375 | 0.342 |
0.386 | |||
1000mg/L | 0.236 | 0.221 | 0.188 |
0.206 |
Precision
We measured random samples of EA0113Ra within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 111.2 | 5.3 | 4.7 |
Intra-assay | 2 | 18 | 175.7 | 10.9 | 6.2 |
Intra-assay | 3 | 18 | 259.1 | 18 | 7 |
Protocols
References
Customer reviews and Q&As