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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0211Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.148	2.193	2.138
	2.238
24ng/ml	1.509	1.445	1.39
	1.381
12ng/ml	0.689	0.723	0.668
	0.756
6ng/ml	0.351	0.36	0.305
	0.37
3ng/ml	0.193	0.177	0.122
	0.16
0ng/ml	0.055	0.055	0
	0.055

We measured random samples of E0211Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	28.6	1.2	4.2
Intra-assay	2	18	10.4	0.5	5.3
Intra-assay	3	18	16.5	0.7	4.1

> Protocol Booklet

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