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Home > Product > ELISA Kit > Human H2A Histone Family, H2AFX ELISA Kit

Human H2A Histone Family, H2AFX ELISA Kit

Human H2A Histone Family, H2AFX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.032ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.032ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

H2A Histone Family

Code

E7195Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.032ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Research area

Epigenetics and Nuclear Signaling

Target protein

H2AFX

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E7195Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.258 2.248 2.224
2.237
8ng/ml 1.42 1.314 1.29
1.208
4ng/ml 0.715 0.746 0.722
0.777
2ng/ml 0.404 0.357 0.333
0.31
1ng/ml 0.151 0.182 0.157
0.212
0ng/ml 0.017 0.024 0
0.031

Precision

We measured random samples of E7195Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 7.5 0.3 3.4
Intra-assay 2 18 4.8 0.2 4.2
Intra-assay 3 18 3 0.2 5.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E7195Hu

    $320.00

  • 96T

    E7195Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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