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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E2288Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160mg/ml	2.067	2.002	1.926
	1.938
80mg/ml	1.122	1.134	1.057
	1.145
40mg/ml	0.554	0.57	0.493
	0.585
20mg/ml	0.322	0.315	0.238
	0.309
10mg/ml	0.17	0.183	0.106
	0.195
0mg/ml	0.072	0.077	0
	0.081

We measured random samples of E2288Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	53.8	2.8	5.2
Intra-assay	2	18	71.9	3	4.2
Intra-assay	3	18	131.2	4.5	3.5

> Protocol Booklet

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