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Home > Product > ELISA Kit > Fish Heat Shock Protein 70, HSP-70 ELISA Kit

Fish Heat Shock Protein 70, HSP-70 ELISA Kit

Fish Heat Shock Protein 70, HSP-70 ELISA Kit

Product Summary

Size:

Sensitivity: 0.096ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Fish

Product Summary

Size:

Sensitivity: 0.096ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Fish

Product overview

Full product name

Heat Shock Protein 70

Size

Sensitivity

0.096ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Code

EA0011FI

Assay type

Competitive

Species

Fish

Storage

2-8°C

Background

Inducible heat shock protein 70 (HSP70) is a stress protein whose expression is upregulated when the cell or organism is placed under conditions of stress. HSP70 is essential for cellular recovery, survival, and maintenance of normal cellular function. It is also a molecular chaperone that prevents protein aggregation and refolds damaged proteins in response to cellular stress caused by environmental insults, pathogens, and disease. Current research is aimed at exploiting HSP70’s cellular protective abilities as a therapeutic strategy against damaging cellular stress.

Research area

Signal Transduction

Target protein

HSP70

Components

Components Quantity
Pre-coated Plate 12 * 8 well strips x 1
Standard, lyophilized 2 vials
Standard/Sample Diluent 6ml ×1 vial
Biotinylated Antigen, lyophilized 1 vial
Avidin-HRP Concentrate 100μl × 1 vial
Biotinylated Antigen Diluent 6ml × 1 vials
Avidin HRP Diluent 5.9ml × 1 vials
Substrate Solution A 6ml ×1 vial
Substrate Solution B 6ml ×1 vial
Stop Solution 6ml ×1 vial
Wash Buffer Concentrate (25x) 20ml × 1 vial

Protocol data

These standard curves of EA0011FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
0ng/ml 2.115 2.16 2.129
2.204
2.5ng/ml 1.627 1.607 1.576
1.587
5ng/ml 1.151 1.096 1.065
1.041
10ng/ml 0.78 0.756 0.725
0.733
20ng/ml 0.409 0.38 0.349
0.35
40ng/ml 0.196 0.21 0.179
0.225

Precision

We measured random samples of EA0011FI within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 22.3 1.1 4.9
Intra-assay 2 18 2.7 0.2 7.7
Intra-assay 3 18 6.1 0.4 6.4

Protocols

References

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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