Product overview
Full product name
Size
Sensitivity
Detection range
Sample type
Code
Assay type
Species
Storage
Background
Inducible heat shock protein 70 (HSP70) is a stress protein whose expression is upregulated when the cell or organism is placed under conditions of stress. HSP70 is essential for cellular recovery, survival, and maintenance of normal cellular function. It is also a molecular chaperone that prevents protein aggregation and refolds damaged proteins in response to cellular stress caused by environmental insults, pathogens, and disease. Current research is aimed at exploiting HSP70’s cellular protective abilities as a therapeutic strategy against damaging cellular stress.
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated Plate | 12 * 8 well strips x 1 |
| Standard, lyophilized | 2 vials |
| Standard/Sample Diluent | 6ml ×1 vial |
| Biotinylated Antigen, lyophilized | 1 vial |
| Avidin-HRP Concentrate | 100μl × 1 vial |
| Biotinylated Antigen Diluent | 6ml × 1 vials |
| Avidin HRP Diluent | 5.9ml × 1 vials |
| Substrate Solution A | 6ml ×1 vial |
| Substrate Solution B | 6ml ×1 vial |
| Stop Solution | 6ml ×1 vial |
| Wash Buffer Concentrate (25x) | 20ml × 1 vial |
Protocol data
These standard curves of EA0011FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 0ng/ml | 2.115 | 2.16 | 2.129 |
| 2.204 | |||
| 2.5ng/ml | 1.627 | 1.607 | 1.576 |
| 1.587 | |||
| 5ng/ml | 1.151 | 1.096 | 1.065 |
| 1.041 | |||
| 10ng/ml | 0.78 | 0.756 | 0.725 |
| 0.733 | |||
| 20ng/ml | 0.409 | 0.38 | 0.349 |
| 0.35 | |||
| 40ng/ml | 0.196 | 0.21 | 0.179 |
| 0.225 |
Precision
We measured random samples of EA0011FI within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 22.3 | 1.1 | 4.9 |
| Intra-assay | 2 | 18 | 2.7 | 0.2 | 7.7 |
| Intra-assay | 3 | 18 | 6.1 | 0.4 | 6.4 |
Protocols
Customer reviews and Q&As
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