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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0064FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.684	1.787	1.728
	1.889
12ng/ml	0.96	0.972	0.914
	0.985
6ng/ml	0.454	0.474	0.415
	0.493
3ng/ml	0.21	0.205	0.146
	0.2
1.5ng/ml	0.112	0.095	0.036
	0.078
0ng/ml	0.059	0.059	0
	0.059

We measured random samples of E0064FI within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	6.5	0.3	4
Intra-assay	2	18	18.7	0.7	3.8
Intra-assay	3	18	13.5	0.7	5

> Protocol Booklet

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